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1

Determination of Serine Hydroxymethyltransferase and Reduced Folate Pools in Tissue Extracts

Dong Woon Kim, Sonia Delle Fratte, Soon-Seog Jeong, Verne Schirch

Journal:

Analytical Biochemistry

Year:

1997

Language:

english

File:

PDF, 160 KB

english, 1997

2

Enzymatic Determination of Homocysteine in Cell Extracts

Tzu-Fun Fu, Martino di Salvo, Verne Schirch

Journal:

Analytical Biochemistry

Year:

2001

Language:

english

File:

PDF, 81 KB

english, 2001

3

Distribution of B6 Vitamers in Escherichia coli as Determined by Enzymatic Assay

Tzu-Fun Fu, Martino di Salvo, Verne Schirch

Journal:

Analytical Biochemistry

Year:

2001

Language:

english

File:

PDF, 137 KB

english, 2001

4

Serine hydroxymethyltransferase revisited

Verne Schirch, Doletha ME Szebenyi

Journal:

Current Opinion in Chemical Biology

Year:

2005

Language:

english

File:

PDF, 280 KB

english, 2005

5

Structure and mechanism of Escherichia coli pyridoxine 5′-phosphate oxidase

Martino L. di Salvo, Martin K. Safo, Faik N. Musayev, Francesco Bossa, Verne Schirch

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2003

Language:

english

File:

PDF, 723 KB

english, 2003

6

Crystal Structure of human pyridoxal kinase: Structural basis of M+ and M2+ activation

Faik N. Musayev, Martino L. di Salvo, Tzu-Ping Ko, Amit K. Gandhi, Ashwini Goswami, Verne Schirch, Martin K. Safo

Journal:

Protein Science

Year:

2007

Language:

english

File:

PDF, 653 KB

english, 2007

7

Site-Directed Mutagenesis in the Study of Serine Hydroxy methyltransf erase

Elena Fattori, Sebastiana Angelaccio, Stefano Pascarella, Francesco Bossa, Donatella Barra, Verne Schirch, Corrisp M. Brunori

Journal:

Rendiconti Lincei

Year:

1990

Language:

english

File:

PDF, 405 KB

english, 1990

8

X-ray structure of Escherichia coli pyridoxine 5′-phosphate oxidase complexed with FMN at 1.8 Å resolution

Martin K Safo, Irimpan Mathews, Faik N Musayev, Martino L di Salvo, Daniel J Thiel, Donald J Abraham, Verne Schirch

Journal:

Year:

2000

Language:

english

File:

PDF, 761 KB

english, 2000

9

Serine Transhydroxymethylase from Rabbit Liver : Sequence of a Nonapeptide at the Pyridoxal-5′-Phosphate-Binding Site

Francesco BOSSA, Donatella BARRA, Filippo MARTINI, La Verne SCHIRCH, Paolo FASELLA

Journal:

Year:

1976

Language:

english

File:

PDF, 418 KB

english, 1976

10

Serine hydroxymethyltransferase : Effect of proteases on the activity and structure of the cytosolic enzyme

Verne SCHIRCH, Douglas SCHIRCH, Filippo MARTINI, Francesco BOSSA

Journal:

Year:

1986

Language:

english

File:

PDF, 672 KB

english, 1986

11

The Function of Arginine 363 as the Substrate carboxyl-binding Site in Escherichia coli Serine Hydroxymethyltransferase

Sonia Delle Fratte, Sandra Iurescia, Sebastiana Angelaccio, Francesco Bossa, Verne Schirch

Journal:

Year:

1994

Language:

english

File:

PDF, 757 KB

english, 1994

12

Role of Cytosolic Serine Hydroxymethyltransferase in One-Carbon Metabolism inNeurospora crassa

Soon-Seog Jeong, Verne Schirch

Journal:

Archives of Biochemistry and Biophysics

Year:

1996

Language:

english

File:

PDF, 173 KB

english, 1996

13

A Noncatalytic Tetrahydrofolate Tight Binding Site Is on the Small Domain of 10-Formyltetrahydrofolate Dehydrogenase

Tzu-Fun Fu, Bruno Maras, Donatella Barra, Verne Schirch

Journal:

Archives of Biochemistry and Biophysics

Year:

1999

Language:

english

File:

PDF, 111 KB

english, 1999

14

Deamidation of Asparagine Residues in a Recombinant Serine Hydroxymethyltransferase

Martino L. di Salvo, Sonia Delle Fratte, Bruno Maras, Francesco Bossa, H.Tonie Wright, Verne Schirch

Journal:

Archives of Biochemistry and Biophysics

Year:

1999

Language:

english

File:

PDF, 299 KB

english, 1999

15

Tight Binding of Pyridoxal 5′-Phosphate to Recombinant Escherichia coli Pyridoxine 5′-Phosphate Oxidase

Emily S. Yang, Verne Schirch

Journal:

Archives of Biochemistry and Biophysics

Year:

2000

Language:

english

File:

PDF, 80 KB

english, 2000

16

The Role of Serine Hydroxymethyltransferase Isozymes in One-Carbon Metabolism in MCF-7 Cells as Determined by 13C NMR

Tzu-Fun Fu, Jason P. Rife, Verne Schirch

Journal:

Archives of Biochemistry and Biophysics

Year:

2001

Language:

english

File:

PDF, 140 KB

english, 2001

17

Diffraction Grade Crystals of Escherichia coli Serine Hydroxymethyltransferase

Patrick Stover, Heidi Kruschwitz, Verne Schirch, H.Tonie Wright

Journal:

Journal of Molecular Biology

Year:

1993

File:

PDF, 181 KB

1993

18

X-ray structure of Escherichia coli pyridoxine 5′-phosphate oxidase complexed with pyridoxal 5′-phosphate at 2.0 Å resolution

Martin K Safo, Faik N Musayev, Martino L di Salvo, Verne Schirch

Journal:

Journal of Molecular Biology

Year:

2001

Language:

english

File:

PDF, 465 KB

english, 2001

19

Active site structure and stereospecificity of Escherichia coli pyridoxine-5′-phosphate oxidase

Martino L di Salvo, Tzu-Ping Ko, Faik N Musayev, Samanta Raboni, Verne Schirch, Martin K Safo

Journal:

Journal of Molecular Biology

Year:

2002

Language:

english

File:

PDF, 618 KB

english, 2002

20

Crystallization and Preliminary X-ray Crystallographic Analysis of Pyridoxine 5′-Phosphate Oxidase Complexed with Flavin Mononucleotide

Faik N. Musayev, Martin K. Safo, Martino L. Di Salvo, Verne Schirch, Donald J. Abraham

Journal:

Journal of Structural Biology

Year:

1999

Language:

english

File:

PDF, 112 KB

english, 1999

21

Purification and Characterization of Recombinant Rabbit Cytosolic Serine Hydroxymethyltransferase

Martino L. di Salvo, Sonia Delle Fratte, Daniela De Biase, Francesco Bossa, Verne Schirch

Journal:

Protein Expression and Purification

Year:

1998

Language:

english

File:

PDF, 159 KB

english, 1998

22

Expression, Purification, and Characterization of RecombinantEscherichia coliPyridoxine 5′-Phosphate Oxidase

Martino Di Salvo, Emily Yang, Genshi Zhao, Malcolm E. Winkler, Verne Schirch

Journal:

Protein Expression and Purification

Year:

1998

Language:

english

File:

PDF, 135 KB

english, 1998

23

Interaction of folylpolyglutamates with enzymes in one-carbon metabolism

Verne Schirch, William B. Strong

Journal:

Archives of Biochemistry and Biophysics

Year:

1989

Language:

english

File:

PDF, 947 KB

english, 1989

24

Similarity between serine hydroxymethyltransferase and other pyridoxal phosphate-dependent enzymes

Stefano Pascarella, Verne Schirch, Francesco Bossa

Journal:

Year:

1993

Language:

english

File:

PDF, 606 KB

english, 1993

25

Properties of human and rabbit cytosolic serine hydroxymethyltransferase are changed by single nucleotide polymorphic mutations

Tzu-Fun Fu, Sharyn Hunt, Verne Schirch, Martin K. Safo, Bing-Hung Chen

Journal:

Archives of Biochemistry and Biophysics

Year:

2005

Language:

english

File:

PDF, 320 KB

english, 2005

26

Kinetic and structural studies of the role of the active site residue Asp235 of human pyridoxal kinase

Amit K. Gandhi, Mohini S. Ghatge, Faik N. Musayev, Aaron Sease, Samuel O. Aboagye, Martino L. di Salvo, Verne Schirch, Martin K. Safo

Journal:

Biochemical and Biophysical Research Communications

Year:

2009

Language:

english

File:

PDF, 318 KB

english, 2009

27

Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases

Martino L. di Salvo, Sharyn Hunt, Verne Schirch

Journal:

Protein Expression and Purification

Year:

2004

Language:

english

File:

PDF, 405 KB

english, 2004

28

[Methods in Enzymology] Vitamins and Coenzymes Part K Volume 281 || Purification of folate-dependent enzymes from rabbit liver

Year:

1997

Language:

english

File:

PDF, 892 KB

english, 1997

29

Synthesis of (6S)-5-formyltetrahydropteroyl-polyglutamates and interconversion to other reduced pteroylpolyglutamate derivatives

Patrick Stover, Verne Schirch

Journal:

Analytical Biochemistry

Year:

1992

Language:

english

File:

PDF, 884 KB

english, 1992

30

Structure and properties of recombinant human pyridoxine 5′-phosphate oxidase

Faik N. Musayev, Martino L. Di Salvo, Tzu-Ping Ko, Verne Schirch, Martin K. Safo

Journal:

Protein Science

Year:

2003

Language:

english

File:

PDF, 426 KB

english, 2003

31

The Role of a Critical Sulfhydryl Group in the Mechanism of Serine Hydroxymethyltransferase

GEETA JOSHI-TOPE, VERNE SCHIRCH

Journal:

Annals of the New York Academy of Sciences

Year:

1990

Language:

english

File:

PDF, 373 KB

english, 1990

32

In vitro conversion of formate to serine: effect of tetrahydropteroylpolyglutamates and serine hydroxymethyltransferase on the rate of 10-formyltetrahydrofolate synthetase

Strong, William B., Schirch, Verne

Journal:

Year:

1989

Language:

english

File:

PDF, 1.35 MB

english, 1989

33

Properties of Tetrahydropteroylpentaglutamate Bound to 10-Formyltetrahydrofolate Dehydrogenase †

Kim, Dong Woon, Huang, Teng, Schirch, Douglas, Schirch, Verne

Journal:

Year:

1996

Language:

english

File:

PDF, 729 KB

english, 1996

34

Serine transhydroxymethylase: evidence for a sequential random mechanism

Schirch, La Verne, Tatum, Charles M., Benkovic, Stephen J.

Journal:

Year:

1977

Language:

english

File:

PDF, 1.22 MB

english, 1977

35

The metabolic role of leucovorin

Stover, Patrick, Schirch, Verne

Journal:

Trends in Biochemical Sciences

Year:

1993

Language:

english

File:

PDF, 590 KB

english, 1993

36

Enzymic mechanism for the hydrolysis of 5,10-methenyltetrahydropteroylglutamate to 5-formyltetrahydropteroylglutamate by serine hydroxymethyltransferase

Stover, Patrick, Schirch, Verne

Journal:

Year:

1992

Language:

english

File:

PDF, 1.85 MB

english, 1992

37

Role of peptide conformation in the rate and mechanism of deamidation of asparaginyl residues

Lura, Richard, Schirch, Verne

Journal:

Year:

1988

Language:

english

File:

PDF, 2.25 MB

english, 1988

38

Interaction of tetrahydropteroylpolyglutamates with two enzymes from mitochondria

Strong, William B., Cook, Robert, Schirch, Verne

Journal:

Year:

1989

Language:

english

File:

PDF, 1.19 MB

english, 1989

39

Thermodynamic Analysis of the Binding of the Polyglutamate Chain of 5-Formyltetrahydropteroylpolyglutamates to Serine Hydroxymethyltransferase †

Huang, Teng, Wang, Changqing, Maras, Bruno, Barra, Donatella, Schirch, Verne

Journal:

Year:

1998

Language:

english

File:

PDF, 96 KB

english, 1998

40

Serine hydroxymethyltransferase: mechanism of the racemization and transamination of D- and L-alanine

Shostak, Keith, Schirch, Verne

Journal:

Year:

1988

Language:

english

File:

PDF, 1.02 MB

english, 1988

41

Serine hydroxymethyltransferase: origin of substrate specificity

Angelaccio, Sebastiana, Pascarella, Stefano, Fattori, Elena, Bossa, Francesco, Strong, William, Schirch, Verne

Journal:

Year:

1992

Language:

english

File:

PDF, 1.04 MB

english, 1992

42

Progress in Bioorganic Chemistry. Vol. 1 (Kaiser, E. T.; Kezdy, F. J.)

Schirch, La Verne

Journal:

Journal of Chemical Education

Year:

1972

Language:

english

File:

PDF, 944 KB

english, 1972

43

Role of Tyrosine 65 in the Mechanism of Serine Hydroxymethyltransferase †

Contestabile, Roberto, Angelaccio, Sebastiana, Bossa, Francesco, Wright, H. Tonie, Scarsdale, Neel, Kazanina, Galina, Schirch, Verne

Journal:

Year:

2000

Language:

english

File:

PDF, 167 KB

english, 2000

44

Serine Hydroxymethyltransferase: Role of Glu75 and Evidence that Serine Is Cleaved by a Retroaldol Mechanism †

Szebenyi, Doletha M. E., Musayev, Faik N., di Salvo, Martino L., Safo, Martin K., Schirch, Verne

Journal:

Year:

2004

Language:

english

File:

PDF, 474 KB

english, 2004

45

Evidence for the accumulation of a stable intermediate in the nonenzymic hydrolysis of 5,10-methenyltetrahydropteroylglutamate to 5-formyltetrahydropteroylglutamate

Stover, Patrick, Schirch, Verne

Journal:

Year:

1992

Language:

english

File:

PDF, 1016 KB

english, 1992

46

Corrigendum

Thomas, Neil R., Schirch, Verne, Gani, David

Journal:

Journal of the Chemical Society Chemical Communications

Year:

1990

File:

PDF, 45 KB

1990

47

Synthesis of (2R)- and (2S)-[1-13C]-2-amino-2-methylmalonic acid, probes for the serine hydroxymethyltransferase reaction: stereospecific decarboxylation of the 2-pro-R carboxy group with the retention of configuration

Thomas, Neil R., Schirch, Verne, Gani, David

Journal:

Journal of the Chemical Society Chemical Communications

Year:

1990

Language:

english

File:

PDF, 309 KB

english, 1990

48

Stereochemical course of the decarboxylation of 2-amino-2-methylmalonic acid by serine hydroxymethyltransferase

Thomas, Neil R., Schirch, Verne, Gani, David

Journal:

Journal of the Chemical Society Perkin Transactions 1

Year:

1993

Language:

english

File:

PDF, 878 KB

english, 1993

49

Molecular basis of E. coli l-threonine aldolase catalytic inactivation at low pH

Remesh, Soumya G., Ghatge, Mohini S., Ahmed, Mostafa H., Musayev, Faik N., Gandhi, Amit, Chowdhury, Nadia, di Salvo, Martino L., Kellogg, Glen E., Contestabile, Roberto, Schirch, Verne, Safo, Martin K

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2015

Language:

english

File:

PDF, 1.45 MB

english, 2015

50

Structure-Based Mechanism for Early PLP-Mediated Steps of Rabbit Cytosolic Serine Hydroxymethyltransferase Reaction

Di Salvo, Martino L., Scarsdale, J. Neel, Kazanina, Galina, Contestabile, Roberto, Schirch, Verne, Wright, H. Tonie

Journal:

BioMed Research International

Year:

2013

Language:

english

File:

PDF, 2.49 MB

english, 2013